FIG 2.
Screening of the anti-DENV activity of C. jagus isolated Amaryllidaceae alkaloids (AAs). (A) Structures of the nine AAs isolated from bulbs of C. jagus. Cherylline-type alkaloids comprised cherylline, gigantelline, and gigantellinine, while crinine types comprised crinine, gigancrinine, and flexinine. Lycorine, sanguinine, and hippadine were also isolated. (B) Anti-DENV activity of AAs. Huh7 cells were treated with compounds (AAs or DMSO vehicle) for 2 h and then infected with DENVGFP at an MOI of 0.15 for 72 h. Infectivity was visualized as GFP+ cells on an inverted microscope system with ×5 and ×20 (hippadine) objectives. The experiment was performed in triplicates at two dilutions. At least 3 pictures were taken from each well. One representative picture of the lowest dilution is displayed. Cher, cherylline; Giga, gigantelline; Gign, gigantellinine; Crin, crinine; Gigc, gigancrinine; Flex, flexinine; Lyco, lycorine; Sang, sanguinine; Hipp, hippadine.