FIG 3.
Cherylline displays anti-DENV activity. (A) Impact of cherylline treatment on DENVR2A (purple) and ZIKVR2A (pink) replication was tested in Huh7.5 cells (MOI, 0.001), and viral-dependent luciferase luminescence was measured at 48 hpi. Cell viability (ATP) was assessed at 48 h. Results are displayed as fold changes in viability and replication, with 1 meaning no change compared to matched concentrations of DMSO-treated cells. (B) Treatment of Huh7 with cherylline dampened infectivity of DENVGFP replication (MOI, 0.15) in a dose-dependent manner, as measured by flow cytometry 72 hpi. (Left) representative dot plots; (right) percentage of infected Huh7 cells using 0.6 to 100 μM cherylline and 10 to 500 μM ribavirin as a positive control. The nonlinear regression curve fit is shown with the 95% of confidence interval in finer lines. (C) Representative pictures of Huh7 treated with different concentrations of cherylline and infected with DENVGFP at an MOI of 0.15. Ribavirin was used as a positive control, and DMSO as a negative control. Infected cells were visualized on an inverted microscope system at 72 hpi.