FIG 4.

Cherylline displays antiviral activity against DENV and ZIKV. (A) Viral titers were measured by plaque assay on Vero E6 cells using the cytopathic WT DENV2 16881s strain. Huh7.5 cells were infected and treated 2 hpi with compounds. Then, 48 h later, supernatants were harvested and plated on Vero E6 cells (left). Fold changes in viability (ATP) were calculated at 48 hpi in Huh7.5 cells (right). (B) Viral titers were measured as in panel A on Vero E6 cells using cytopathic WT Uganda MR766 ZIKV strains (left) and French Polynesia H/PF/2013 (right). (C) THP-1 cells were treated with two concentrations of each compound in triplicates for 2 h and infected with VSV-G pseudotyped HIV-1GFP at an MOI of 1. The antiretroviral nevirapine was used as a positive control, and DMSO (vehicle), as negative a control. Results were analyzed by flow cytometry 72 hpi.