FIG 6.
Cytotoxicity and antiviral effects of cherylline in PBMCs. (A) The cytotoxicity of antiviral concentrations of cherylline in PBMCs was measured by ATP production and normalized over DMSO at 48 h posttreatment. Means with SEM are shown. Dotted lines represent the concentration used in the antiviral assay. Ch, cherylline. (B) Antiviral activity of cherylline in PBMCs. PBMCs were infected with DENVGFP preincubated with anti-Envelope antibody 4G2 at an MOI of 2, treated with cherylline (30 μM) or DMSO (0.05%), and analyzed 72 hpi by flow cytometry. The experiment was performed in triplicates (uninfected) and six-plicates (infected). Representative plots are shown.