TABLE 1.
EC50, CC50, and selectivity index of AAs as calculated with GraphPad Prisma
| Alkaloids | CC50 (μM) | EC50 (μM) for |
SI |
||
|---|---|---|---|---|---|
| DENVR2A | ZIKVR2A | DENVR2A | ZIKVR2A | ||
| Lycorine | 14.5 | 0.16 | 0.41 | 90.6 | 35.4 |
| Cherylline | >250 | 8.8 | 20.33 | >28 | >12.3 |
| Hippadine | 175.9 | 73.8 | 114 | 2.38 | 1.54 |
| Flexinine | >250 | 63.4 | 216.3 | >3.9 | >1.15 |
| Gigantellinine | >250 | 104.6 | >250 | >2.3 | ND |
a
EC50 was determined using DENV/ZIKVR2A infection from 0.05 to 25 μM for lycorine and NITD008 and from 0.5 to 250 μM for the others as in Fig. 2A. CC50 was calculated using the same concentrations in the same cell type, Huh7.5 at 48 h posttreatment. CC, cytotoxic concentration; EC, effective concentration; SI, therapeutic selectivity index; ND, not detected.