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Proof of PfgyrA disruption. PCR with different primers helped validate disruption of the PfgyrA gene. Genetically modified parasites yielded a larger PCR fragment when amplified with primers P1/P2. Amplification with primers P1/P3 and P4/P2 further confirmed the insertion of the bsd gene in the modified parasites. SP, signal peptide; TP, transit peptide; HR, homologous region.
