FIG 2.

Effect of buparvaquone (BPQ) exposure on Sporothrix brasiliensis CBS 133006 strain. (A) Scanning electron microscopy images of yeasts treated with 0.08 μg/ml BPQ showed alterations in cell wall integrity and cell disruption (arrows and insets in image ii). Yeasts analyzed by flow cytometry exhibited a significant decrease in mitochondrial activity (B), increased cytoplasmic ROS (C), an increase in neutral lipid (D), and a disrupted plasma membrane (E). Scale bars, 2 μm and 200 nm (insets). *, P < 0.05; ****, P < 0.0001 by Student's t test. Data represent means ± standard errors of the means. a.u, arbitrary units.