TABLE 2.
MICs of antifungals against TBCZ31, TBCZ33, TBCZ34, and TBCZ37
| Strain | Genotype | MIC (μg/ml) ofa: |
Source or description | |||
|---|---|---|---|---|---|---|
| ITCZ | VRCZ | PSCZ | AMPH | |||
| ΔpyrG strain | akuA::loxP ΔpyrG | 0.25 | 0.5 | 0.063 | 1 | This study, derived from AfS35 |
| TBCZ31 | akuA::loxP ΔpyrG mfsDR337LagcAE535Stop | 2 | 4 | 0.25 | 1 | |
| TBCZ33 | akuA::loxP ΔpyrG mfsDR337LagcAE535Stop | 2 | 4 | 0.5 | 1 | |
| TBCZ34 | akuA::loxP ΔpyrG mfsDR337LubcDT98KabcJG297E | >8 | 4 | 1 | 1 | |
| TBCZ37 | akuA::loxP ΔpyrG mfsDR337LrttAA83T | 2 | 4 | 0.25 | 1 | |
| TBCZ15 | akuA::loxP ΔpyrG mfsDR337L | 0.5 | 2 | 0.125 | 1 | |
| AfS35 | akuA::loxP | 0.25 | 0.5 | 0.063 | 0.5 | Fungal Genetics Stock Center (strain A1159) |
| ΔmfsD strain | akuA::loxP mfsD::hph | 0.25 | 0.5 | 0.063 | 0.5 | This study |
| ΔagcA strain | akuA::loxP agcA::hph | 0.25 | 1 | 0.063 | 0.5 | This study |
| rttAwt strain | akuA::loxP hph | 0.25 | 0.25 | 0.032 | 1 | hph gene cassette was inserted between Afu7g04735 and rttA |
| rttAA83T strain | akuA::loxP rttAA83Thph | 0.25 | 0.5 | 0.063 | 1 | rttA was replaced with rttAA83T; hph gene cassette was inserted between Afu7g04735 and rttA |
a
MICs were determined at 48 h after inoculation.