TABLE 3.
Bacterial strains used in this study
| Strain | Description | Source or reference |
|---|---|---|
| S. aureus USA300 | USA300 LAC, cured of resistance plasmids | Lab stock |
| S. aureus RN4220 | rK− mK+; capable of accepting foreign DNA | Lab stock |
| R1 | 6-TG-resistant S. aureus mutant | This study |
| R3 | 6-TG-resistant S. aureus mutant | This study |
| R5 | 6-TG-resistant S. aureus mutant | This study |
| R6 | 6-TG-resistant S. aureus mutant | This study |
| R7 | 6-TG-resistant S. aureus mutant | This study |
| R8 | 6-TG-resistant S. aureus mutant | This study |
| R9 | 6-TG-resistant S. aureus mutant | This study |
| R10 | 6-TG-resistant S. aureus mutant | This study |
| R11 | 6-TG-resistant S. aureus mutant | This study |
| R1 pEmpty | R1 carrying pALC2073 | This study |
| R1 pstgP | R1 carrying the wild-type version of stgP from S. aureus | This study |
| R10 pEmpty | R10 carrying pALC2073 | This study |
| R10 phpt | R10 carrying the wild-type version of hpt from S. aureus | This study |
| RN4220 pEmpty | RN4220 carrying pALC2073 | 24 |
| RN4220 ptgs | RN4220 carrying the 6-TG biosynthetic operon from S. chromogenes ATCC 43764 in pALC2073 | 24 |