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. 2020 Sep 16;2(3):229–237. doi: 10.1089/bioe.2019.0037

FIG. 3.

FIG. 3.

The use of differentiated drNPC monolayer as a substrate elicits anodal migration of undifferentiated drNPCs under an applied EF. (a) Directed migration of differentiated, but not undifferentiated, drNPCs in the presence of an EF. (b) Experimental set-up of combinations of substrates with cell monolayers. (c) Fluorescent image of the cell monolayer plated with DiI-labeled, undifferentiated drNPCs (arrows). Scale bar = 100 μm. (d) Directedness of DiI-labeled drNPCs in the presence or absence of an EF when plated on a cell monolayer (on matrigel or fibronectin) or plated on the surface of a fibronectin-coated cell monolayer. Each bar represents ≥45 cells from ≥3 independent experiments for each group. Means were compared by using Kruskal–Wallis test followed by Dunn's correction for multiple pairwise comparisons. Data are represented as means ± SEM. (e) Representative plots of individual cell migration paths for drNPCs plated on a cell monolayer (n = 45 cells) under an applied EF. Each line represents one cell. (f) Hoescht-labeled nuclei (blue) of a cell monolayer, with and without a fibronectin coated surface. Scale bar = 100 um. ****p < 0.0001.