FIG. 3.
Collagen-PPy hydrogel microfibers improve PC12 neurogenesis. (A, B) Immunofluorescent staining of Tubulin-β3 (red), L-VGCC (green), and DAPI (blue) of PC12 cells on several compositions of Collagen-PPy hydrogels, Arrows indicate immunostaining of Tubulin-β3 and L-VGCC. (C, D) Semiquantitation of Tubulin-β3 and L-VGCC expression. (E–G) Normalized fold changes of gene expression of Tubulin-β3, Cacnb3, and NF-66. * indicate significance at p < 0.05, n = 3. Adapted from Wu et al.86 with permission from American Chemical Society. PPy, polypyrrole.