Figure 3.

Mis-splicing of neurodegeneration-causing genes. (A) The OMIM database was used to select, among the genes that are mis-spliced in both human and mouse striatum, genes whose mutations in humans cause monogenic forms of neurodegeneration. (B) Using VastDB,²⁸ the orthologous alternative spliced events differentially processed in both species were identified and the PSI (per cent spliced in) values of the mis-spliced events in each gene are shown in three wild-type versus three R6/1 samples (according to the RNA-seq vast-tools analysis). (C) RT-PCR assay and quantification of mis-spliced events in striatal RNA from an independent set of wild-type and R6/1 mice (n = 5). (Student’s t-test; *P < 0.05, **P < 0.01). Data represent mean ± SEM.