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. 2021 Aug 5;17(8):e1009848. doi: 10.1371/journal.ppat.1009848

Fig 1. Mapping of gK binding to SPP.

Fig 1

A) Schematic diagram of gK constructs used for binding to SPP. Full-length gK is shown at the top. gK1, gK2, and gK3 represent the 1st, 2nd, and 3rd regions of full-length gK, respectively. gK1.1 is similar to the gK1 fragment except that it is lacking its 30 aa signal sequence. All constructs have an ATG and a termination codon (TAA) and are inserted into pcDNA3.1 with 3X in-frame Flag tags. B) Binding of gK to SPP in vitro. HeLa cells were transfected with Flag-gK1 or Flag-gK1.1 and HA-SPP plasmids at a 1:1 ratio for 48 hr. Cell lysates were incubated with anti-Flag antibody bound to IgG beads and the resulting IP was analyzed by western blot using anti-HA antibody. Lower blot shows similar SPP expression in all three samples using anti-HA antibody for the western blot.