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. 2021 Aug 5;17(8):e1009848. doi: 10.1371/journal.ppat.1009848

Fig 2. gK4 peptide inhibits the binding of gK protein to SPP protein.

Fig 2

A) Blocking gK binding to SPP. HEK293 cells were co-transfected with Flag-gK and HA-SPP plasmids. Cells were harvested 48 hr after transfection. Cell lysate was incubated with the indicated peptide for 2 hr at 4°C. IP using anti-Flag antibody in the presence of 5 μg/ml of indicated peptide recovered similar amounts of Flag-gK protein, but significantly less HA-SPP was recovered in the presence of peptide 4. B) Mutated form of gK4 does not precipitate SPP. HEK293 cells were transfected with HA-SPP and Flag-gK or Flag-gK mutant (amino acid RCIYA was mutated to AAAAA). Cell lysates were immunoprecipitated using anti-Flag antibody. The Flag-gK4 mutant failed to pull down HA-SPP, indicating the importance of the mutated region for gK binding to SPP. Panels: A) Mapping gK binding region to SPP; and B) Fine mapping of gK4 binding to SPP.