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. 2021 Aug 5;17(8):e1009848. doi: 10.1371/journal.ppat.1009848

Table 1. Fine mapping of the gK binding region to SPP using gK synthetic peptidesa.

# Peptide aa region Binds to SPP
1 MLAVRSLQHLSTVVL 1–15 No
2 STVVLITAYGLVLVW 10–25 No
3 LVLVWYTVFGASPLH 20–35 No
4 ASPLHRCIYAVRPTG 30–45 Yes
5 VRPTGTNNDTALVWM 40–55 No
6 ALVWMKMNQTLLFLG 50–65 No
7 LLFLGAPTHPPNGGW 60–75 No
8 PNGGWRNHAHICYAN 70–85 No
9 ICYANLIAGRVVPFQ 80–95 No
10 VVPFQVPPDAMNRRI 90–105 No
11 MNRRIMNVHEAVNCL 100–115 No
12 AVNCLETLWYTRVR 110–125 No
13 TRVRLVVVGWFLYLA 120–135 No
14 FLYLAFVALHQRRCM 130–145 No
15 QRRCMFGVVSPAHKM 140–155 No
16 PAHKMVAPATYLLNY 150–165 No
17 YLLNYAGRIVSSVFL 160–175 No
18 SSVFLQYPYTKITRL 170–185 No
19 KITRLLCELSVQRQN 180–195 No
20 VQRQNLVQLFETDPV 190–205 No
21 ETDPVTFLYHRPAIG 200–215 No
22 RPAIGVIVGCELMLR 210–225 No
23 ELMLRFVAVGLIVGT 220–235 No
24 LIVGTAFISRGACAI 230–245 No
25 GACAITYPLFLTITT 240–255 No
26 LTITTWCFVSTIGLT 250–265 No
27 TIGLTELYCILRRGP 260–275 No
28 LRRGPAPKNADKAAA 270–285 No
29 DKAAAPGRSKGLSGV 280–295 No
30 GLSGVCGRCCSIILS 290–305 No
31 SIILSGIAVRLCYIA 300–315 No
32 LCYIAVVAGVVLVAL 310–325 No
33 VLVALHYEQEIQRRL 320–335 No

aA panel of 33 overlapping HSV-1 gK peptides (15-mers with five-amino-acid overlaps) based on the full-length 338 amino acids of gK was used to fine map the region of gK that binds to SPP as we described previously [9,14].