FIG 1.

CFU and mucin levels following V. cholerae infections in a zebrafish host. CFU/ml levels of V. cholerae in the zebrafish intestines (A) and in the infection water 24 hours postinfection (B). The data include the results from 2 to 8 individual experiments. Fish, n = 8 to 16; water, n = 2 to 6. A total of 50-ml water samples were spun down and resuspended in 1 ml of PBS and then analyzed for mucin concentrations using Schiff’s reagent (C) and Bradford protein assay reagent (D). n = 2 to 6. Statistical tests were performed with Dunnett's multiple-comparison test (A and B) and Tukey’s multiple-comparison test (C and D) on log-transformed data.*, P ≤ 0.05; **, P ≤ 0.005; ***, P ≤ 0.0005; ****, P ≤ 0.0001.