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Study design and EPEC colonization. (A) Mouse pups were challenged at P7 (EPEC, ΔescV, or LB broth [sham]), and adults were divided into 3 experimental groups. (B) Colonic (left) and ileum (right) samples grown on MacConkey agar plates at 1, 4, 8, and 12 days postinfection to quantify EPEC and ΔescV colonization. Data represent 1 to 2 pups per litter from 3 to 4 separate litters per group per time point.
