FIG 6.

Neonatal EPEC infection increases hippocampal neuroinflammation in adulthood. (A) Relative mRNA expression in the hippocampus (n = 12 to 16 mice per group) (*, P ≤ 0.05; **, P ≤ 0.01 [by Student’s t test, with a Mann-Whitney test and Welch’s correction where appropriate]). (B) Confocal imaging and morphological characterization of microglia in the dentate gyrus (DG) (top) and CA1 region (bottom) of the hippocampus. (Left) Microglia (Iba1) (green) with single-cell overlays (red) identified with 3DMorph and morphology characterized using Imaris. (Right) Process lengths, terminal points, branch points, and cell numbers were quantified. Nuclei were stained with DAPI (n = 5 to 6 mice per group) (**, P < 0.01; ***, P < 0.001 [by Student’s t test]). Bars = 15 μm.