Table 1: Concentration of protein reconstituted into liposomes composed of DPPC or 9:1 DPPC:DPPS (molar ratio).
The protein and lipid concentration were determined as described in Materials and Methods. Proteoliposomes contained AnxA5 or TNAP, or both, as indicated.
| Proteoliposomes | [Protein] incorporated (μg protein/mg lipid) | ||
|---|---|---|---|
| Lipid specificity | Protein content | ||
| AnxA5 | TNAP | ||
| DPPC | + | − | 29.0 ± 2.3 |
| − | + | 30.8 ± 3.3 | |
| + | + | 40.2 ± 4.2 | |
| 9:1 DPPC:DPPS | + | − | 30.7 ± 3.5 |
| − | + | 30.0 ± 2.3 | |
| + | + | 38.5 ± 3.2 | |