Table 2:
Effect of collagen type and matrix composition on binding of proteoliposomes harboring AnxA5 and TNAP+AnxA5. A total of 90 μg lipid was deposited per well, both for DPPC and 9:1 DPPC:DPPS proteoliposomes.
| Proteoliposomes | Different collagens sources (binding %) | ||||||
|---|---|---|---|---|---|---|---|
| Lipid content | Protein content | ||||||
| AnxA5 | TNAP | Type II | Type I + III | TypeI | Native from VSMCs | Native from osteoblast/chondrocyte-like cells | |
| DPPC | + | − | 21.5 ± 2.7 | 10.3 ± 0.8 | 7.3 ± 0.6 | 6 ± 0.8 | 13 ± 0.6 |
| + | + | 12.9 ± 1.5 | 12.7 ± 1.0 | 13.4 ± 1.1 | 8 ± 0.9 | 17 ± 0.9 | |
| 9:1 DPPC:DPPS | + | − | 73.2 ± 4.8 | 40.6 ± 2.4 | 16.5 ± 1.3 | 95 ± 2.3 | 81 ± 4.0 |
| + | + | 29.3 ± 0.4 | 1.3 ± 0.5 | 0.8 ± 0.5 | 73 ± 2.4 | 29 ± 1.5 | |