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. Author manuscript; available in PMC: 2021 Aug 17.
Published in final edited form as: Biochim Biophys Acta Gen Subj. 2020 Apr 29;1864(8):129629. doi: 10.1016/j.bbagen.2020.129629

Table 2:

Effect of collagen type and matrix composition on binding of proteoliposomes harboring AnxA5 and TNAP+AnxA5. A total of 90 μg lipid was deposited per well, both for DPPC and 9:1 DPPC:DPPS proteoliposomes.

Proteoliposomes Different collagens sources (binding %)
Lipid content Protein content
AnxA5 TNAP Type II Type I + III TypeI Native from VSMCs Native from osteoblast/chondrocyte-like cells
DPPC + 21.5 ± 2.7 10.3 ± 0.8 7.3 ± 0.6 6 ± 0.8 13 ± 0.6
+ + 12.9 ± 1.5 12.7 ± 1.0 13.4 ± 1.1 8 ± 0.9 17 ± 0.9
9:1 DPPC:DPPS + 73.2 ± 4.8 40.6 ± 2.4 16.5 ± 1.3 95 ± 2.3 81 ± 4.0
+ + 29.3 ± 0.4 1.3 ± 0.5 0.8 ± 0.5 73 ± 2.4 29 ± 1.5