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. 2021 Sep;27(9):1082–1101. doi: 10.1261/rna.078782.121

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© 2021 Douka et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 2. — Association of lncRNAs polysomes during neuronal differentiation. Volcano plots displaying the significantly differentially localized lncRNAs (labeled by their geneIDs) between Total and Polysome populations in (A) Control and (B) RA, with log₂ fold-change cutoff = 1, P^adj < 0.05. (C–E) RT-qPCR of lncRNAs across sucrose gradient fractions (n = 3, SE is plotted). (C) LINC02143 is found in 80S and small polysome fractions during differentiation. Five percent of the transcript is detected in 80S (monosome) fractions and 66% in small polysome complexes. (D) DLGAP1-AS2 is found in 80S and small polysome fractions both in control and RA treated cells. On average, 63% of the transcript is detected in the polysome fractions in Control and 49% upon differentiation. (E) LINC01116 is found in 80S and two to seven polysome fractions both in control and RA treated cells. On average, 66% of the LINC01116 transcript is detected in the polysome fractions in Control and 57% upon differentiation.