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. 2021 Sep;27(9):981–990. doi: 10.1261/rna.078758.121

FIGURE 4.

FIGURE 4.

N state stabilization upon Kan or Par binding to PRE complex. Kan (A) and Par (B) effect on the PRE complex dynamics in the absence of EF-G. Body (teal) and head (pink) FRET fluorescence time courses were monitored in the stopped-flow apparatus upon addition of Kan (30 µM) or Par (5 µM) to PRE complexes. (C) Concentration dependence of kapp values on Kan (0.1–100 µM). kapp values were estimated by single-exponential fitting of time courses as in A. Body (closed symbols) and head (open symbols). Lines show hyperbolic fits. Error bars (which are smaller than symbol site) represent the error of the fit.