Fig. 2.7.

Fluorescent Filter Cube. Components typically contained in a fluorescent cube; Exciter filter, Dichroic Mirror, and Barrier Filter. These work together to ensure that the only frequency that makes it to the photo recorder is that of the fluorescent tag being excited, plus whatever autofluorescence is present in that channel (Modified from ‘Fluorescence Filters’, [https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/fluorescence/filters/]. Image courtesy of Olympus Corporation. Reprinted with permission)