Figure 2. SBI-0206965 inhibits unstimulated and 991-stimulated glucose uptake with no apparent inhibition of TBC1D1 Ser231 phosphorylation in C2C12 myotubes.

(A,B) C2C12 myotubes were pre-incubated for 30 min with vehicle (0.1% DMSO) or the indicated concentrations of SBI-0206965 followed by an additional 1 h incubation with 991 (10 μM) or vehicle. Cell lysates were subjected to immunoblot analysis using the indicated antibodies. Tubulin was used as a loading control. Representative images (A) and quantification of the indicated blots (B) from three independent experiments are shown (n = 3 per treatment). (C) C2C12 myotubes were incubated with vehicle or the indicated compounds for 1 h followed by addition of [³H]-2-deoxy-glucose and measurement of glucose uptake for 5 min (n = 8–9 per treatment). Results are expressed as mean ± SEM. *P < 0.05 (vehicle vs. indicated compound(s) treated).