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Primer sequences for qRT-PCR.
| Gene | Primer (5′ to 3′) | |
|---|---|---|
| ZO-1 | Forward | CACCTCGCACGCATCACAGC |
| Reverse | GGCGGCAATGGTGGTCCTTC | |
| Occludin | Forward | TTGGCTACGGAGGTGGCTATGG |
| Reverse | CCTTTGGCTGCTCTTGGGTCTG | |
| Claudin-1 | Forward | TGGCTTCTCTGGGATGGATCGG |
| Reverse | CCTGAGCGGTCACGATGTTGTC | |
| Claudin-2 | Forward | CCGTGTTCTGCCAGGATTCTCG |
| Reverse | AGCCCAGGATGCCACCAAGG | |
| GAPDH | Forward | GGTTGTCTCCTGCGACTTCA |
| Reverse | TGGTCCAGGGTTTCTTACTCC |
