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. 2021 Aug 13;16:5479–5494. doi: 10.2147/IJN.S313339

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© 2021 Jin et al.

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(A) In vitro cytotoxicity of 4T1 cells treated with different formulations. 4T1 cell viability after treatment with different NPs for 48 h (N/P = 30, C/N = 1/5, PTX content: 6.04%, 20 nM of siRNA per well). **p < 0.01 compared with PBS Control, Blank NPs, and siRNA^N.C-NPs. (B) 4T1 cell viability after treatment with siRNA and PTX simultaneously by complex NPs. The concentration of PTX varied from 0.05 to 5 µg/mL, and the concentration of VEGF siRNA was 20 nM. **p < 0.01 compared with PTX. Data are presented as the mean ± SEM of quintuplicate experiments.