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. 2021 Aug 17;12(9):799. doi: 10.1038/s41419-021-04081-0

Fig. 2. LINC00671 inhibits proliferation, migration, and invasion by downregulating the expression of LDHA in thyroid cancer cells.

Fig. 2

A TPC-1 cells were transfected with LINC00671 or LINC00671 plus LDHA expression vector. The cell proliferation was determined by the CCK-8 assay. The representative immunoblot shows the LDHA expression. The histogram shows the expression of LINC00671 as detected by qRT-PCR. B Colony formation assay of TPC-1 cells transfected as in (A). Representative image shows the colonies in plates (upper panels). The histogram shows colony number. C, D Wound healing (C) and Transwell assays (D) of TPC-1 cells transfected as in (A). Right histograms show relative cell migration and invasion. E, F Lentivirus-mediated LDHA knockdown (LDHA shRNA) or control TPC-1 cells were transfected with Scramble or LINC00671 smart pool of silencers and analyzed as in (A) and (B). G, H Wound healing (G) and Transwell (H) assays of lentivirus-mediated LDHA knockdown (LDHA shRNA) or control TPC-1 cells were transfected as in (E and F). Scale bar, 50 μm. All values shown are mean ± SD of triplicate measurements and have been repeated three times with similar results (*P < 0.05, **P < 0.01 versus corresponding control).