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. 2021 Aug 17;12(9):799. doi: 10.1038/s41419-021-04081-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A TPC-1 cells were transfected with LINC00671 or LINC00671 plus LDHA expression vector. Glucose uptake and the production of lactate and ATP were determined. Representative immunoblot reveals the expression of LDHA. qRT-PCR analysis indicates the LINC00671 expression. B, C TPC-1 cells were transfected as in (A), and extracellular acidification rate (ECAR) (B) and oxygen consumption rate (OCR) (C) were then evaluated. LDHA shRNA or control TPC-1 cells were transfected with Scramble or LINC00671 smart pool of silencers. D Glucose uptake, lactate production, and ATP production were measured. Typical immunoblot reveals the expression of LDHA. qRT-PCR analysis shows the LINC00671 expression. E, F ECAR (E) and OCR (F) assays of LDHA knockdown or control TPC-1 cells were transfected as in (D). All values shown are mean ± S.D. of triplicate measurements and have been repeated three times with similar results (A–F). *P < 0.05 versus corresponding NC. **P < 0.01 versus corresponding NC.