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. 2021 Aug 17;12(9):799. doi: 10.1038/s41419-021-04081-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A TPC-1 cells were transfected with LINC00671 smart pool of silencers and treated with Niclocide. The proliferation of the cells was detected by CCK-8 assay. The representative immunoblot shows pSTAT3 level. Histograms show LINC00671 expression determined by qRT-PCR. B Colony formation assay of TPC-1 cells transfected and treated as in (A). Representative images show colonies in plates (upper panels). Histograms show colony number. C, D Wound healing (C) and Transwell (D) assays of TPC-1 cells transfected and treated as in (A). Right histograms show the relative cell migration and invasion. E Glucose uptake and the production of lactate and ATP were determined. Representative immunoblot reveals the expression of pSTAT3. qRT-PCR analysis indicates LINC00671 expression. F, G TPC-1 cells were transfected and treated as in (A), and extracellular acidification rate (ECAR) (F), and oxygen consumption rate (OCR) (G) were then measured. The displayed values are the mean ± standard deviation. The triplicate measurement results were repeated 3 times and the results were similar. *P < 0.05, **P < 0.01 versus the corresponding control. Scale bar, 50 μm.