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. 2021 Aug 17;11:16666. doi: 10.1038/s41598-021-96150-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Photoreceptor segment lengths in hypoxia. (a) Representative retinal morphologies and (b) graph showing the ratio (%) of rod photoreceptor segment length to outer nuclear layer (ONL) thickness for indicated hypoxic conditions. (c) Representative immunostaining for cone arrestin (ARR3, green) and (d) the percentage of cone photoreceptor segment length to outer nuclear layer (ONL) thickness. Cell nuclei were counterstained with DAPI (grey). One-way ANOVA with Dunnett’s multiple comparison test was used to compare each condition to control (normoxia) *p < 0.05; ***p < 0.001; ****p < 0.0001. RPE: retinal pigment epithelium; PS: photoreceptor segments; ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer. Scale bars as indicated.