FIGURE 3.

Effect of CTX on the formation of tubule-like structures in 3D-matrigel and migratory behavior on type 2D-type I collagen evaluated in Time Lapse. CTX-pretreated t.End.1 cells were added on polymerized Matrigel in 96-well plates and incubated for 6 h. The results are expressed in a number of tubular structures and represent the count of five fields from triplicates of each group. The data are presented from three distinct experiments. *p < 0.0001, compared to the control group (A). Photomicrograph of representative tube formation. Images obtained at the ×40 objective (B). For the 2D-assay, time lapse images obtained from CTX pretreated t.End.1 cells migrating under collagen coating in In Cell Analyzer GE 2200 equipment in a ×10 objective for 18 h. The t.End.1 cell velocity (C), relative distance (D), and directionality (E) were measured. These parameters were evaluated using Image J software. Data are mean ± s.e.m. *p < 0.05 by comparison with the control group (n = 29) and CTX (n = 33). The graphs were plotted from the data collected during three independent experiments.