KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| rat anti-CD31, clone SZ31 | Dianova | Cat#DIA-310; RRID:AB_2631039 |
| rat anti-CD8, clone 4SM15 | Thermo fisher) | Cat#14–0808-82; RRID: AB_2572861 |
| rabbit anti-PanCK, polyclonal | Novus Biologicals | Cat#NBP2–44368 |
| anti-mouse CD45-BV650, clone 30F11,1:400 | Biolegend | Cat#103151; RRID: AB_2565884 |
| anti-mouse CD3e-PECy5.5,clone 145–2C11, 1:100 | Invitrogen | Cat#;35–0031-80; RRID: AB_11218085 |
| anti-mouse CD4-PB, clone GK1.5, 1:100 | Biolegend | Cat#100428; RRID: AB_493647 |
| anti-mouse CD8-BV711, clone 53.6.7, 1:50 | Biolegend | Cat#100748; RRID: AB_2562100 |
| anti-mouse CD11b-PECy7, clone M1/70,1:200 | eBioscience | Cat#25–0112-81; RRID: AB_469587 |
| anti-mouse CD11c-BV60, clone N418 | Biolegend | Cat#117334; RRID: AB_2562415 |
| anti-mouse F4/80-APCCy7, clone BM8,1:200 | Biolegend | Cat#123118; RRID: AB_893477 |
| anti-mouse F4/80-Alexa647, clone F4/80,1:50 | home-made | |
| anti-mouse PD-L1-BV711, clone 10F.9G2,1:200 | Biolegend | Cat#124319; RRID: AB_2563619 |
| anti-mouse Ki67-APC, clone 16A8, 1:200 | Biolegend | Cat#652406; RRID: AB_2561930 |
| anti-mouse CD80-PEDazzle,clone 16–10A1, 1:100 | Biolegend | Cat#104737; RRID: AB_2564174 |
| anti-mouse CD86-APCCy7, clone GL-1,1:100 | Biolegend | Cat#105030; RRID: AB_2244452 |
| anti-mouse CD103-PE, clone 2E7, 1:200 | Biolegend | Cat#121405; RRID: AB_535948 |
| Anti-Mouse CD16/CD32 (Mouse BD Fc Block), Clone 2.4G2, 1:100 | BD | Cat#553142; RRID:AB_394657 |
| LIVE/DEAD Fixable Aqua Dead Cell Stain, 1:500 | Invitrogen | Cat#L34957 |
| anti-human BRCA1, clone MS110, 1:500 | Millipore | Cat#MABC199; RRID: AB_213438 |
| anti-human IFI16, clone 1G7, 1:500 | Santa Cruz Biotechnology | Cat#sc-8023; RRID: AB_627775 |
| anti-human and mouse NF-κB, clone C-20, 1:500 | Santa Cruz Biotechnology | Cat#sc-372; RRID: AB_632037 |
| anti-human and mouse phosphor-IRF3, clone 4D4G, 1:500 | Cell Signaling Technology | Cat#4947S;,RRID: AB_823547 |
| anti-human and mouse STING, clone D2P2F, 1:500 | Cell Signaling Technology | Cat#13647S; RRID: AB_2732796 |
| anti-human and mouse phospho-STAT1, clone D4A7, 1:500 | Cell Signaling Technology | Cat#7649S; RRID: AB_10950970 |
| anti-human and mouse STAT1, clone D4Y6Z, 1:500 | Cell Signaling Technology | Cat#14995S; RRID: AB_2716280 |
| anti-human and mouse Lamin B, clone M-20, 1:1000 | Santa Cruz Biotechnology | Cat#sc-6217; RRID: AB_648158 |
| anti-human and mouse B-ACTIN, clone C4, 1:500 | Santa Cruz Biotechnology | Cat#sc-47778; RRID: AB_2714189 |
| dsDNA Marker, HYB331–01, 1:1000 | Santa Cruz Biotechnology | Cat#sc-58749; RRID: AB_783088 |
| anti-phospho-Histone H2A.X (Ser139), clone JBW301, 1:500 | Millipore | Cat#05–636; RRID: AB_309864 |
| anti-human and mouse phospho-TBK1/NAK(Ser172), clone D52C2, 1:100 | Cell Signaling Technology | Cat#5483; RRID: AB_10693472 |
| anti-human CD8, clone SP16, 1:100 | ThermoFisher Scientific | Cat#MA5–14548; RRID: AB_10984334 |
| anti-human and mouse STAT1, 1:2000 | Abcam | Cat#ab47425; RRID: AB_882708 |
| anti-human and mouse phospho-STAT1, Clone 58D6 | Cell Signaling Technology | Cat#9167S; RRID: AB_561284 |
| anti-human pan Cytokeratin, clone AE1/AE3 | DAKO | Cat#M351501–2; RRID: AB_2631307 |
| anti-human and mouse phospho-Stat1-AF488, clone 58D6, 1:100 | Cell Signaling Technology | Cat#9174S; RRID: AB_2198287 |
| anti-human and mouse phospho-TBK1-AF647,clone D52C2, 1:100 | Cell Signaling Technology | Cat#14590S; RRID: AB_2798527 |
| InVivoMAb anti-mouse CTLA-4, clone 9D9 | BioXCell | Cat#BE0164; RRID: AB_10949609 |
| InVivoMAb anti-mouse PD-L1, clone 10F.9G2 | BioXCell | Cat#BE0101; RRID: AB_10949609 |
| anti-VEGFA mAb, clone B20–4.1.1 | Genentech | a kind gift from Genentech |
| InVivoMAb anti-mouse IFNAR-1, clone MAR1–5A3 | BioXCell | Cat#BE0241; RRID: AB_2687723 |
| InVivoMAb IgG1 Isotype control, clone MOPC-21 | BioXCell | Cat#BE0083; RRID: AB_1107784 |
| H3K27ac pAb | Active Motif | Cat#31933 |
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| Biological samples | ||
|
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| HGSOC specimens | (Bernards et al., 2016; Pennington et al., 2014); https://doi.org/10.1016/j.ygyno.2015.12.017 https://doi.org/10.1158/1078–0432.CCR-13–2287 | |
| ovarian carcinomas TCGA Consortium | TCGA | (Cancer Genome Atlas Research Network, 2011); https://doi.org/10.1038/nature10166 |
|
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| Chemicals, peptides, and recombinant proteins | ||
|
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| Spectral DAPI, 1:5 | PerkinElmer | Cat#FP1490 |
| IgG Goat Blocker | Ventana Roche | Cat#760–6008 |
| DISCOVERY OmniMap anti-rabbit HRP | Ventana Roche | Cat#760–4311 |
| DISCOVERY OmniMap anti-mouse HRP | Ventana Roche | Cat#760–4310 |
| OPAL 520 | PerkinElmer | Cat#FP1487001KT |
| OPAL 570 | PerkinElmer | Cat#FP1488001KT |
| OPAL 620 | PerkinElmer | Cat#FP1495001KT |
| OPAL 690 | PerkinElmer | Cat#FP1497001KT |
| Olaparib, AZD2281 | Selleckchem | Cat#S1060 |
| poly(dA:dT) | Invivogen | Cat# tlrl-patn, tlrl-patn-1 |
| poly(I:C) | Invivogen | Cat#tlrl-pic |
| Turbofect | ThermoFischer Scientific | Cat#R0532 |
| D-luciferin | Biosynth | Cat#L-8220 |
| Liberase TL | Roche | Cat#540102001 |
| Dnase I | Sigma Aldrich | Cat#D4527 |
| Hygromycin | Millipore | Cat#400052 |
| Puromycin | Invivogen | Cat#ant-pr-1 |
| TRIzol reagent | Invitrogen | Cat#15596026 |
| DMEM | ThermoFischer Scientific | Cat#41966–029 |
| RPMI 1640 | ThermoFischer Scientific | Cat#1870010 |
| Opti-MEM I Reduced Serum Medium, no phenol red | ThermoFischer Scientific | Cat#11058021 |
| TaqMan® Fast Universal PCR reagents | Applied Biosystems | Cat#4366072 |
| Shandon Shandon-Mount | ThermoFischer Scientific | Cat#1900331 |
|
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| Recombinant DNA | ||
|
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| MISSION pLKO.1-puro non-target shRNAcontrol | Sigma | Cat#SHC016 |
| BRCA1 MISSION shRNA | Sigma | Cat#TRCN0000244984 |
| TMEM173 MISSION shRNA Bacterial Glycerol Stock transmembrane protein 173 (STING kd1) | Sigma | Cat#TRCN0000346266 |
| TMEM173 MISSION shRNA Bacterial Glycerol Stock transmembrane protein 173 (STING kd2) | Sigma | Cat#TRCN0000346320 |
| CCL5 MISSION shRNA Bacterial GlycerolStock chemokine (C-C motif) ligand 5 | Sigma | Cat#TRCN0000068102 |
| MSCV Luciferase PGK-hygro constrcuct | Addgene | Cat#18782 |
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| Commercial assays | ||
|
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| Human IFNa Flex Set | BD | Cat#560379 |
| Human IL-1a Flex Set | BD | Cat#560153 |
| Human IL-1b Flex Set | BD | Cat#561509 |
| Human TNFa Flex Set | BD | Cat#561516 |
| Human CXCL10 Flex Set | BD | Cat#558280 |
| Human Fractalkine (CX3CL1) Flex Set | BD | Cat#560265 |
| Human IL-6 Flex Set | BD | Cat#558276 |
| Human GM-CSF Flex Set | BD | Cat#558335 |
| Mouse RANTES (CCL5) Flex Set | BD | Cat#558345 |
| MTT Cell Proliferation Assay kit | R&D Systems | Cat#4890–025-K |
| RNA Easy Mini Kit | QIAGEN | Cat#74104 |
| PrimeScript First Strand cDNA Synthesis Kit | Takara | Cat#6110A |
| NE-PER Nuclear and cytoplasmic Extraction Kit | ThermoFischer Scientific | Cat#78833 |
| Halt Protease and Phosphatase Inhibitor Cocktail (100X) | ThermoFischer Scientific | Cat#78440 |
| NucleoSpin Gel and PCR-Clean-up | Macherey-Nagel | Cat#740609.50 |
| Illumina TruSeq Stranded Total RNA reagents | Illumina | Cat#RS-122–2201 |
| Illumina HiSeq PE Cluster Kit v4 cBot reagents | Illumina | Cat#PE-401–400 |
| HiSeq SBS Kit V4 reagents | Illumina | Cat#FC-401–4002 |
| Mouse VEGF-A ELISA Kit | Abcam | Cat#ab209882 |
|
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| Deposited data | ||
|
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| ChIPSeq data | GEO | GSE122155; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE122155 |
| HiC data | GEO | GSE122155; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE122155 |
| Human RNA sequencing data | GEO | GSE120792; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE120792 |
| Mouse RNA sequencing data | GEO | GSE162935; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE162935 |
|
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| Experimental models: Cell lines | ||
|
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| OVCAR5 BRCA1wt and OVCAR5 BRCA1kd | our own laboratory | |
| UWB1.289 (ATCC® CRL-2945), BRCA1mut | ATCC | RRID:CVCL_B079 |
| UWB1.289 BRCA1+ (ATCC® CRL-2946), BRCA1wt | ATCC | RRID:CVCL_B078 |
| ID8 Trp53−/− and ID8 Trp53−/−Brca1−/− | Prof. Iain A. McNeish lab | (Walton et al., 2016; Walton et al., 2017); https://doi.org/10.1158/0008–5472. CAN-16–1272 https://doi.org/10.1038/s41598–017-17119–1 |
|
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| Experimental models: Organisms/strains | ||
|
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| C57/BL6 | Envigo | 057 |
|
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| Oligonucleotides | ||
|
| ||
| CCL5 | Applied Biosystems | Cat#Hs00982282_m1 |
| CXCL9 | Applied Biosystems | Cat#Hs00171065_m1 |
| CXCL10 | Applied Biosystems | Cat#Hs01124251_g1 |
| CD8A | Applied Biosystems | Cat#Hs00233520_m1 |
| IFNB1 | Applied Biosystems | Cat#Hs01077958_s1 |
| IFNA1 | Applied Biosystems | Cat#Hs00256882_s1 |
| TNFA | Applied Biosystems | Cat#Hs01113624_g1 |
| ISG20 | Applied Biosystems | Cat#Hs00158122_m1 |
| ISG15 | Applied Biosystems | Cat#Hs01921425_s1 |
| VEGFA | Applied Biosystems | Cat#Hs03929036_s1 |
| VEGFB | Applied Biosystems | Cat#Hs00173634_m1 |
| VEGFC | Applied Biosystems | Cat#Hs01099203_m1 |
| GAPDH | Applied Biosystems | Cat#Hs03929097_g1 |
| Cd8a | Applied Biosystems | Cat#Mm01188922_m1 |
| Ccl5 | Applied Biosystems | Cat#Mm01302427_m1 |
| Cxcl9 | Applied Biosystems | Cat#Mm00434946_m1 |
| Stat1 | Applied Biosystems | Cat#Mm01257286_m1 |
| Gzmb | Applied Biosystems | Cat#Mm00442837_m1 |
| Ifng | Applied Biosystems | Cat#Mm01168134_m1 |
| Itgax | Applied Biosystems | Cat#Mm00498701_m1 |
| Batf3 | Applied Biosystems | Cat#Mm01318275_m1 |
| Ifnb1 | Applied Biosystems | Cat#Mm00439552_s1 |
| Gapdh | Applied Biosystems | Cat#Mm99999915_g1 |
| Vegfa | Applied Biosystems | Cat#Mm00437306_m1 |
| Vegfb | Applied Biosystems | Cat#Mm00442102_m1 |
| Vegfc | Applied Biosystems | Cat#Mm00437310_m1z |
| Tnfa | Applied Biosystems | Cat#Mm00443258_m1 |
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| Software and algorithms | ||
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| GraphPad Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| R | Open source | https://www.r-project.org/ |