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. 2021 Mar 9;246(11):1274–1286. doi: 10.1177/1535370221997071

Figure 1.

Figure 1.

miR-31 knockdown induces abnormal motor neuron outgrowth. Tg (hb9:EGFP) zebrafish embryos were injected with 8 ng of control or miR-31 MO. (a–h) Representative bright field and fluorescent images of Tg(hb9:EGFP) embryos at 48 hpf. (b–d) Image of trunk regions taken at 48 hpf, with the spinal motor neurons structures visualized by eGFP fluorescence and labeled motor axon (white arrows), showed normal development in the embryo injected with the control MO. Compared with the control MO, embryos injected with miR-31 MO present a lower number of incomplete motor axons (f–h). The percentage of embryos with spinal motor neurons defects (i). The bar graph shows the percentage of embryos with motor axon defects. The stereotypic escape response was abnormal in miR-31 morphants at 48 hpf. (j) Quantification of the number of complete motor axons shows a significant decrease in miR-31 MO-injected embryos. Columns, mean; bars, SEM (n = 10; unpaired student’s t-test), ***P < 0.0001. hpf: hours post-fertilization. (A color version of this figure is available in the online journal.)