Figure 5.

The mRNA expression of Nestin, Math1, ChAT, Neurog, HB9, and GFAP after miR-31 transfection of NSCs. (a) Expression of miR-31 according to the agomir/antagomir treatments. (b) Western blot was used to detect the changes in the protein expression of Nestin, ChAT, and GFAP (markers of NSCs, neurons, and glial cells) after the transfection of miR-31. (c) Immunofluorescence was used to detect the differentiation of cells into NSCs after miR-31 transfection. (A color version of this figure is available in the online journal.)