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Effects of WISP1 expression on TGF-β-Smad signaling in LSCC cells. (a) TU212 cells were transfected with WISP1 vectors or si-WISP1, then the protein levels of TGF-β, p-Smad2, Smad2, p-Smad3, and Smad3 were analyzed by Western blotting. (b–d) The quantified analysis on binding bands was evaluated by Image J software. *P < 0.05.
