Table 2.
Primers used in this study.
| Primer | Sequence (5ꞌ → 3ꞌꞌ) | Restriction site |
|---|---|---|
| pME-gfp-S | AAA GGTACC ATGAG TAAAG GAGAA GAACT | KpnI |
| pME-gfp-A | AAA AGATCT TTATT TGTAT AGTTC ATCCA | BglII |
| pME-zapAL-gfp-S | AAAAGAATTCATGAGCCGCGACGGCGTCCA | EcoRI |
| pME-zapAL-gfp-A | AAAA GGTACC GCCGGA TGCCCC GGCGAT | KpnI |
| pME-zapA-gfp-S | AAA GAATTC ATGAG CCAGT CGAAT ACCCT | EcoRI |
| pME-zapA-gfp-A | AAAA GGTACC GCCGGA TGCCCC GGCGAT | KpnI |
| pME-zapAL-S | AAAA GGTACC ATGAG CCGCG ACGGC GTCCA | KpnI |
| pME-zapAL-A | AAAA AGATCT CTAGCC GGATGC CCCGGC GAT | BglII |
| pEX-zapAL-upstream-S | ATAGGTACCAACGAACTCGTTCGGCGGCT | KpnI |
| pEX-zapAL-upstream-A | GGTTCTAGATGGCTCATGTGTCCTGCTCC | XbaI |
| pEX-zapAL-downstream-S | AAATCTAGAGATGCCGGCGAAGCCTGACC | XbaI |
| pEX-zapAL-downstream-A | AGTAAGCTTTAGGGGCTGGCGGGATCCAG | HindIII |
| pEX-zapA-upstream-S | ATA GAATTC GTGCGCCAGATCACAAGTTA | EcoRI |
| pEX-zapA-upstream-A | AGG TCTAGA TGTTCTCCAGCATGATCTCG | XbaI |
| pEX-zapA-downstream-S | AAC TCTAGA CCTGGAAGCGCTGGTCGAGC | XbaI |
| pEX-zapA-downstream-A | ATA AAGCTT CTACGAGCTGGATGGCCGCC | HindIII |
Restriction enzyme sites are underlined.