. 2021 Aug 4;12:715712. doi: 10.3389/fmicb.2021.715712

TABLE 5.

Effect of dietary TB on SCFA concentration (μmol/g fresh digesta) in ileal and cecal digesta in broilers.

Item	Bacteria	Control	TB1	TB2	TB3	TB4	Pooled SEM	Treatment P-value	Linear P-value	Quadratic P-value
21 days
Ileal digesta	Acetic acid	6.81^b	7.19^b	7.39^ab	7.52^a	7.98^a	0.05	0.00	0.08	0.04
	Propionic acid	0.81^b	0.84^b	0.88^b	0.89^b	1.01^a	0.01	0.00	0.03	0.03
Cecal digesta	Acetic acid	42.87^b	54.92^a	56.29^a	57.68^a	59.74^a	0.77	0.00	0.41	0.27
	Propionic acid	1.87^c	2.04^bc	2.48^ab	2.69^a	2.73^a	0.05	0.01	0.28	0.03
	Isobutyric acid	0.36^b	0.45^b	0.50^ab	0.51^ab	0.59^a	0.01	0.01	0.16	0.08
	Butyric acid	11.27^b	12.11^b	12.20^b	14.07^ab	15.20^a	0.17	0.02	0.05	0.03
	Isovaleric acid	0.16^c	0.33^b	0.39^b	0.47^b	0.52^a	0.02	0.00	0.24	0.04
	Valeric acid	0.99^b	1.01^b	1.19^ab	1.32^a	1.34^a	0.02	0.00	0.23	0.06
	Total VFAs	65.14^b	78.89^b	81.32^a	85.15^a	89.11^a	1.04	0.00	0.33	0.18
42 days
Ileal digesta	Acetic acid	8.09	8.42	8.64	8.81	8.98	0.04	0.22	0.49	0.21
	Propionic acid	0.76^b	0.81^b	0.84^b	0.89^ab	0.97^a	0.02	0.00	0.03	0.00
Cecal digesta	Acetic acid	42.93^b	44.49^b	45.99^ab	48.25^ab	52.31^a	0.43	0.03	0.01	0.00
	Propionic acid	3.65^b	3.81^ab	3.85^ab	3.96^ab	4.22^a	0.02	0.01	0.03	0.02
	Isobutyric acid	0.76^b	0.81^b	0.87^ab	0.98^ab	1.06^a	0.01	0.04	0.05	0.00
	Butyric acid	11.42^c	12.20^c	13.43^bc	15.06^ab	15.94^a	0.22	0.01	0.10	0.01
	Isovaleric acid	0.86^b	0.93^b	0.98^b	1.18^ab	1.36^a	0.02	0.02	0.02	0.01
	Valeric acid	1.61^b	1.63^b	1.70^ab	1.74^ab	1.83^a	0.01	0.04	0.01	0.02
	Total VFAs	70.08^b	73.10^b	76.30^ab	80.87^a	86.67^a	0.76	0.03	0.01	0.04

SEM, standard error of the mean; TB, tributyrin. Control = basal diet; TB1 = basal diet supplemented with 230 mg/kg TB; TB2 = basal diet supplemented with 460 mg/kg TB; TB3 = basal diet supplemented with 920 mg/kg TB; TB4 = basal diet supplemented with 1,840 mg/kg TB. ^a,b,cMean values within a row with unlike superscript letters were significantly different (P < 0.05).