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. 2021 Aug 4;12:718091. doi: 10.3389/fpls.2021.718091

FIGURE 5.

FIGURE 5

Petal elongation of ray florets was inhibited by transient silencing of Gh14-3-3b and Gh14-3-3f. (A) Petal phenotype of Mock, Gh14-3-3b transient silencing, Gh14-3-3f transient silencing, and those treated with 10 μM BL (Mock, Gh14-3-3b-VIGS, Gh14-3-3f-VIGS, Mock + BL, Gh14-3-3b-VIGS + BL, Gh14-3-3f-VIGS + BL). Bar = 1 cm. (B) The confocal microscope image of epidermal cells in top, middle and basal regions of petals. Bar = 20 μm. (C) The relative expression level of Gh14-3-3b in Mock, Gh14-3-3b-OE, Mock + BL and Gh14-3-3b-VIGS + BL. (D) The relative expression level of Gh14-3-3f in Mock, Gh14-3-3f-OE, Mock + BL, and Gh14-3-3f-VIGS + BL. The length (E) and relative elongation rate (F) of Mock, Gh14-3-3b-VIGS, Gh14-3-3f-VIGS, Mock + BL, Gh14-3-3b-VIGS + BL, and Gh14-3-3f-VIGS + BL petals (n = 45). The elongation rate was calculated at day 8. The cell length (G) and cell number (H) in top, middle, and basal regions in transgenic ray petals. At least three biological replicates were used for each observation.