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. 2021 Aug 4;9:700854. doi: 10.3389/fcell.2021.700854

FIGURE 8.

FIGURE 8

The function of CTRP1 in apoptosis and PERK signal pathway in neurons after OGD/R. (A) The expression of CTRP1 in neurons was analyzed by Western blot. n = 4 per group. (B) qRT-PCR analyzed the mRNA expression of CTRP1 after being transfected with siRNA and overexpression lentivirus, respectively. n = 3 per group. (C) Cell viability of neurons analyzed by MTT, n = 4 per group. (D) Apoptosis was analyzed by TUNEL, n = 3 per group. The representative images were acquired under × 200 magnification, scale bars = 100 μm. (E) The expression of CTRP1, BAX, Bcl-2, Cleaved caspase3, and Caspase 12 was analyzed by Western blot. n = 4 per group. (F) The protein expression of CHOP, GRP78, ATF4, PERK, and p-PERK in neurons. n = 4 per group. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05 vs. control group, ####p < 0.0001, ###p < 0.001, ##p < 0.01, #p < 0.05 vs. OGD/R + si-NC group, @@@@p < 0.0001, @@@p < 0.001, @p < 0.05 vs. OGD/R + LV-NC group.