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. 2021 Aug 4;9:706327. doi: 10.3389/fped.2021.706327

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Copyright © 2021 Xu, Wang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pue inhibited ferroptosis of LPS-induced A549 cells. (A) Iron Assay Kit detected total iron levels in cells in LPS-induced cells when Pue induced. (B) Iron Assay Kit detected Fe²⁺ levels in cells in LPS-induced cells when Pue induced. (C) ROS levels were detected with DCFH-DA fluorescent probe in LPS-induced cells when Pue induced. N = 5. (D) The expression of iron death related proteins was detected by Western blot in LPS-induced cells when Pue induced. N = 3. **P < 0.01 and ***P < 0.001.