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. 2021 Aug 4;9:710729. doi: 10.3389/fcell.2021.710729

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Copyright © 2021 Mimura, Tomita, Sugai, Shinkai, Ishihara and Kurita.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LLPS of mixtures of the oligomers and H1. (A) Turbidimetric titration curves of H1 against solutions containing 5.0 μM of the oligomers. (B) Phase-contrast microscopy images of the solutions under the conditions indicated by the dotted line in panel A (5.0 μM oligomer and 2.5 μM H1); scale bar = 20 μm. (C) Fusion process of the spherical assemblies formed by oligo(pdA) (5.0 μM) and H1 (2.5 μM); scale bar = 10 μm. (D) Left: confocal fluorescence microscopy images during FRAP measurements. White arrowhead indicates the bleached area; scale bar = 10 μm. Right: FRAP recovery curves for the oligomers; values shown represent mean values ± standard deviation (n = 3). The table below lists the fluorescence-recovery times calculated by single-exponential fitting. All experiments were carried out in 10 mM Tris-EDTA buffer (pH = 7.4).