Figure 1.

Btn2a2 is regulated during osteoclastogenesis in a RANKL-dependent manner. (A) Btn2a2 expression was analyzed by quantitative RT-PCR at days 2, 4 and 6 during osteoclast assay from Btn2a2-/- , Btn2a2+/-, and wild-type littermate controls. Relative expression values normalized to the housekeeping gene GAPDH are shown. (B) Quantita­ tive RT-PCR for determination of relative mRNA levels of Btn2a2 normalized on GAPDH, after 2 hours stimulation of osteoclast precursors with indicated RANKL concentrations. Data are representative of 2 independent experiments, with 3 mice per group. Significance was assessed using one- (B) or two-way ANOVA (A) and Bonferroni's post-hoc test. Data are shown as means± SEM. *P < 0.05; **P < 0.01. ****P < 0.0001.