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. 2021 Aug 4;12:685060. doi: 10.3389/fendo.2021.685060

Figure 3.

Figure 3

Loss of Btn22 leds to reduced numbers of bone degrading osteoclasts. (A) quantification of the number of multinucleated TRAP+ osteoclasts from Wild-type, Btn2a2+/- and Btn2a2-/-mice after osteoclast assay. (B) Trap staining of Wild-type and Btn2a2-/- osteoclasts at day 5 of osteoclast assay and (C) quantification of the number of multinucleated TRAP+ osteoclasts by their number of nuclei. (D) violin plots displaying area of multinucleated TRAP+ osteoclasts at day 5 of osteoclast assay of wild-type Btn2a2+/- and Btn2a2-/- mice with each dot representing one osteoclast. (E) Flow cytometric analysis of bone marrow progeni­tor cells of wild-type and Btn2a2-/- mice. (F) OCP migration abilityof wild-type and Btn2a2-/- OCPs was assessed in vitro. Quantification of migrated cells was performed with images captured after 24 hours. Data are representative of two independent experiments, with 3 mice per group. (G) Analysis of resorption acitvity of Btn2a2-/- and their wild-type liltermates after 5 days of culture. (H) Resorbed area per osteoclast. Data are representative of two independent experiments, with 3-4 mice per group (B, C, E–G) or represent two pooled experiments (A, D, H). Significance was assessed using one-way (A) or two-way ANOVA (C) and Bonferro­ni's post-hoc test or using two-tailed Students t-test. (D) Signifiance was assessed using Krusaii-Wallis test and pairwise comparisons using Wilcoxon rank sum test with Bonferroni's p value adjustments. (B) Scale bar indicates 200 μm. Data are shown as means t SEM. *P < 0.05; **P < 0.01; ****P < 0.0001.