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. 2021 Aug 4;12:685060. doi: 10.3389/fendo.2021.685060

Figure 4.

Figure 4

Loss of Btn2a2 changes bone architecture in vivo. (A) Representative Images of tibiae with TRAP staining of male Btn2a2-/- mice compared with their wild type littermates at 12 weeks of age. Scale bar indicates 200 μm. (B) Histomorphometric analysis of the number of osteoclasts per bone perimeter (N.OC/B.Pm) and the osteoclast surface per bone surface (OC.surtace/B.surtace). (C) μCT images of the skeletal phenotype of male Btn2a2-/- mice and their wild-type littermates at 12 weeks of age. Scale bar indicates 1 mm. (D) μCT analysis of trabecular bone parameters of tibial bone including bone volume to trabecular volume (BV/TV), trabecular thickness (Tb.Th.), trabecular number (Tb number), and trabecular separation (Tb.Sp.). (E) Serum levels of CTX-1 and OPG from Btn2a2-/- mice and wild-type littermates at 12 weeks of age were measured by ELISA. (F) BTN2A2 serum levels from RA patients and healthy controls. (G) Btn2a2 serum levels from CIA mice compared to healthy controls at day 30 of CIA. Significance was assessed using Student's t-test. Date are shown as means ± SEM. *P < 0.05; **P < 0.01; ****p < 0.0001.