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. 2021 Aug 4;9:634190. doi: 10.3389/fcell.2021.634190

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Copyright © 2021 Chien, Chien, Lin, Tsai, Chou, Yarmishyn, Rastegari, Wang, Leu, Yang, Wang, Jheng, Lai, Ching, Huo, Cherng and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Preparation and characterization of PU-PEI₆₀₀-Mal complexes loaded with the plasmids encoding the components of CRISPR/Cas9 system. (A) Schematic outline of experimental design showing the structures of two plasmids (top) and workflow of chemical modifications to generate conjugate complexes for transfection (bottom). (B) TEM images of the indicated PU-PEI₆₀₀/DNA complexes. (C) TEM images of a mix of unconjugated PU-PEI₆₀₀-Mal/plasmid1 and PU-PEI₆₀₀-Mal/plasmid2 complexes (top) and the same complexes conjugated by HDT (bottom).