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. 2021 Aug 4;9:634190. doi: 10.3389/fcell.2021.634190

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Copyright © 2021 Chien, Chien, Lin, Tsai, Chou, Yarmishyn, Rastegari, Wang, Leu, Yang, Wang, Jheng, Lai, Ching, Huo, Cherng and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PU-PEI₆₀₀-Mal-HDT-mediated transfection is not cytotoxic for iPSCs. (A) MTT cell viability assay showing growth/viability of Ctrl-iPSCs treated with the indicated formulations of PU-PEI₆₀₀/DNA complexes. Data shown as means from three independent experiments with standard deviation error bars. (B) Bright-field images of Ctrl-iPSCs treated with the indicated PU-PEI₆₀₀/DNA complexes. (C) Comparison of cell viability between untreated Ctrl-iPSCs and Ctrl-iPSCs treated with the indicated PU-PEI₆₀₀/DNA complexes at different time points of cardiac differentiation (days 0, 15, and 30). Data expressed as mean percentages relative to “Untreated” from three independent experiments with standard deviation error bars.