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. 2021 Aug 4;8:696578. doi: 10.3389/fcvm.2021.696578

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Copyright © 2021 Zhang, Zhou, Yuan, Nan and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of gamma-aminobutyric acid type B receptor subunit 2 (GABBR2) knockdown on blood recovery of the ischemic hindlimb in mice. (A) Protocol of adenovirus injection and the establishment of mouse hindlimb ischemia. (B) Knockdown efficiency of GABBR2 in mouse hindlimb. (C) Blood recovery of mouse hindlimb imaged by laser Doppler at different time points. (D) Statistical analysis of the blood perfusion ratio (ischemic/non-ischemic hindlimb). (E) Expression changes of VEGF and CD31 post-ischemia in wild-type (WT) and GABBR2-downregulated hindlimbs. (F) Immunofluorescent staining of CD31 in WT and GABBR2-downregulated ischemic hindlimbs. Bar, 200 μm. *p < 0.05; **p < 0.01.