. 2021 Jul 25;58(3):163–170. doi: 10.2141/jpsa.0200086

Table 1. Plasma free amino acid concentration in chick embryos.

	Control		L-Leu		P value
Amino acids	ED 14	ED 18	ED 14	ED 18	L-Leu	Age	L-Leu×Age
Histidine	90±8	133±21	97±8	137±14	NS	P<0.01	NS
Threonine	483±90	682±34	345±27	746±150	NS	P<0.01	NS
Valine	449±16	611±37	450±39	528±27	NS	P<0.001	NS
Isoleucine	219±10	218±24	235±16	237±5	NS	NS	NS
Leucine	175±9	199±24	199±12	216±14	NS	NS	NS
Phenylalanine	101±5	156±21	105±6	176±12	NS	P<0.001	NS
Methionine	55±4	107±12	56±5	102±9	NS	P<0.001	NS
Lysine	396±28^ab	266±27^b	410±39^ab	527±70^a	P<0.01	NS	P<0.05
Tyrosine	483±9^a	232±33^c	420±33^ab	341±26^b	NS	P<0.0001	P<0.01
Proline	227±10	386±14	199±12	335±20	P<0.05	P<0.0001	NS
Arginine	265±15	308±39	268±6	369±20	NS	P<0.01	NS
Glycine	186±18	180±22	165±16	187±6	NS	NS	NS
Aspartic acid	13±2	16±3	13±4	15±2	NS	NS	NS
Alanine	273±17	318±37	245±18	328±7	NS	P<0.05	NS
Glutamic acid	37±3	118±22	41±2	125±11	NS	P<0.001	NS
Cysteine	43±4	102±12	38±3	94±9	NS	P<0.001	NS
Serine	345±34	637±28	343±32	593±33	NS	P<0.001	NS

Different superscripts in the same row indicate significant differences (P<0.05). The number of embryos used in each group was n=6. Values are represented as mean±SEM in pmol/µL. Control, sterile water injection; L-Leu, L-Leucine injection; ED, embryonic day; NS, not significant.