. 2021 Jul 25;58(3):163–170. doi: 10.2141/jpsa.0200086

Table 2. Hepatic free amino acid concentration in chick embryos.

	Control		L-Leu		P value
Amino acids	ED 14	ED 18	ED 14	ED 18	L-Leu	Age	L-Leu×Age
Histidine	350±27	498±13	372±23	487±16	NS	P<0.0001	NS
Threonine	2749±93	3149±417	2598±148	3514±56	NS	P<0.05	NS
Valine	563±26	772±39	540±28	726±22	NS	P<0.0001	NS
Isoleucine	218±12	244±13	213±5	258±11	NS	P<0.01	NS
Leucine	559±34	681±22	539±28	719±30	NS	P<0.0001	NS
Phenylalanine	320±15	381±13	309±10	385±14	NS	P<0.0001	NS
Methionine	102±13	139±8	98±12	160±10	NS	P<0.001	NS
Lysine	752±45^ab	579±39^b	681±46^ab	806±79^a	NS	NS	P<0.01
Tyrosine	746±22^a	655±15^b	674±39^ab	739±34^ab	NS	NS	P<0.05
Proline	234±25	367±14	205±1	305±5	NS	P<0.001	NS
Arginine	380±21	506±28	367±26	547±34	NS	P<0.0001	NS
Glycine	1228±55	951±10	1185±51	1000±29	NS	P<0.0001	NS
Aspartic acid	2097±39	1310±91	1899±60	1476±114	NS	P<0.0001	P<0.05
Alanine	1094±25	1072±42	960±40	1175±74	NS	NS	P<0.05
Glutamic acid	3071±133	2841±240	2960±106	3153±138	NS	NS	NS
Serine	1510±58	1637±96	1479±34	1650±73	NS	P<0.05	NS

Different superscripts in the same row indicate significant differences (P<0.05). The number of embryos used in each group was n=6. Values are represented as mean±SEM in pmol/mg wet tissue. Control, sterile water injection; L-Leu, L-Leucine injection; ED, embryonic day; NS, not significant.