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. 2021 Aug 18;21:929. doi: 10.1186/s12885-021-08675-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Immunofluorescence of macrophage migration inhibitory factor (MIF) and macrophage markers in NPC tissues. (a) Representative images of the inflammation and NPC images of MIF (red), CD68 (green), merged images of the red and green channels, and nuclei counterstained using DAPI. (b) Graphic presentation of the IF scores for MIF and CD68. (c) Representative images of CD11c (red), CD163 (green), merged images, and DAPI. (d) Graphic presentation of IF scores for CD11c and CD163. “E” indicates epithelia, “non-E” indicates the non-epithelial area, “TN” indicates tumor nest, “TS” indicates tumor stroma. The red cross in the graphic represents the median. *: p < 0.05, **: p < 0.01, ***: p < 0.001 analyzed by unpaired test, ###: p < 0.001 analyzed by paired test. An enlarged image is shown in the inset. Scale bar represents 50 μm